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primer3

primer3 [Biology] Tool to design flanking oligo nucleotides for DNA amplification
Priority
Sectionscience
Installed size 224
Maintainer Debian-Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>
Architecture i386
Version 1.0b-1
Depends libc6 (>= 2.3.6-6)
Suggests ncbi-epcr
File name pool/main/p/primer3/primer3_1.0b-1_i386.deb
Description Primer3 picks primers for PCR reactions, considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair. . It has been published in Rozen S and Skaletsky H, "Primer3 on the WWW for general users and for biologist programmers.", Methods Mol Biol. 2000;132:365-86. . The Whitehead Institute for Biomedical Research provides a web-based front end to Primer3. . Homepage: http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi


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